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TLS Online TPP Program
#Question id: 16134
#Unit 13. Methods in Biology
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.
TLS Online TPP Program
#Question id: 6962
#Unit 5. Developmental Biology
In which way is the organizer region of the sea urchin similar to that in frogs?
TLS Online TPP Program
#Question id: 26829
#Unit 10. Ecological Principles
Natural Enemies Promote
TLS Online TPP Program
#Question id: 23086
#Unit 12. Applied Biology
______________ is an example of a physical method of sterilization,
TLS Online TPP Program
#Question id: 33143
#Unit 2. Cellular Organization
Which of the following protein act as a critical regulator of Cdk substrates during the cell cycle?