TLS Online TPP Program

#Question id: 726


The structure of a protein is known from X-ray diffraction studies which gave 30% - helix, 50% -sheet and 20% random coil. Circular dichroism (CD) measurements gave 50% -helix, 40% -sheet and 10% random coil. What could not be a possible explanation for these observations.

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. Protein structure in the crystal is different from that in the solution.

  2. CD analysis for structural components is not appropriate for this protein.

  3. Contributions from other chromophores also contribute to the CD spectrum of the protein

  4. Protein contains high content of disulphide bonds.