Very low fluence responses such as 0.0001 µmol m–2 reach a maximum at about 0.05 µmol m–2, on these fluence rate red light induce phytochrome responses. Which of the following is not the phytochrome responses on VLFR

TLS Online TPP Program

#Question id: 23176

#Unit 13. Methods in Biology

When we use a high-intensity light to permanently bleach the fluorochrome in the patch, there will initially be no fluorescence and it will look 

TLS Online TPP Program

#Question id: 23177

#Unit 13. Methods in Biology

(FRET) technique uses a pair of fluorescent proteins in which the emission wavelength of the first is 

TLS Online TPP Program

#Question id: 23178

#Unit 13. Methods in Biology

If cyan fluorescent protein (CFP) and yellow  fluorescent protein (YFP) are present in close proximity  and emission light of wavelength 433nm is applied what will be observed.

TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.

1. YFP

2. RFP

3. GFP

4. CFP

Which option below contains correct combination of flurorescent molecules that can give FRET?

TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than 

TLS Online TPP Program

#Question id: 23181

#Unit 13. Methods in Biology

__________ type of biosensors exist to measure the concentration and location of Ca2+ and the activation state of GTPase switch proteins.