As with cryptochrome and phytochrome, the N-terminal photosensory region of the phototropins controls the activity of the C-terminal half of the protein, which contains a serine/threonine kinase domain. Following are some statements about photosensory region of the phototropins.

a) The activation of the C-terminal kinase domain then leads to receptor autophosphorylation on multiple serine residues that is required for all phototropin-mediated responses

b) In the dark, the N-terminal region, including the LOV domains, “cages” and inhibits the activity of the kinase domain

c) In the light, the N-terminal region, including the LOV domains, “cages” and inhibits the activity of the kinase domain

d) Absorption of blue photons by the LOV domains results in primary photochemical changes that lead to the uncaging of the kinase domain and its activation by the unfolding of the Jα-helix

Which one of the following combination of above statements is correct?

TLS Online TPP Program

#Question id: 12923

#Unit 13. Methods in Biology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.

TLS Online TPP Program

#Question id: 12924

#Unit 13. Methods in Biology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

TLS Online TPP Program

#Question id: 12925

#Unit 13. Methods in Biology

Another name of TaqMan assay is ____

TLS Online TPP Program

#Question id: 12926

#Unit 13. Methods in Biology

PCR is undertaken with a fluorescence reporter / quencher pair called______

TLS Online TPP Program

#Question id: 12927

#Unit 13. Methods in Biology

When the ends cut by enzyme are not only identical but complementary and will base pair with each other they are therefore known as _______

TLS Online TPP Program

#Question id: 12928

#Unit 13. Methods in Biology

Name the enzyme which is used to stabilize bases.