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                                TLS Online TPP Program
                                                    #Question id: 23171
#Unit 13. Methods in Biology
Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize
 
                                                    TLS Online TPP Program
                                                    #Question id: 23172
#Unit 13. Methods in Biology
Green fluorescent protein do contains a tripeptide sequence i.e. ser-tyr-gly but it only fluoresce when 
 
                                                    TLS Online TPP Program
                                                    #Question id: 23173
#Unit 13. Methods in Biology
There are various drawbacks still in fluorescence microscopy such as
1.  Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
 
                                                    TLS Online TPP Program
                                                    #Question id: 23175
#Unit 13. Methods in Biology
If we use a high-intensity light on to the fluorochrome there will initially be 
 
                                                    TLS Online TPP Program
                                                    #Question id: 23176
#Unit 13. Methods in Biology
When we use a high-intensity light to permanently bleach the fluorochrome in the patch, there will initially be no fluorescence and it will look 
 
                                                    TLS Online TPP Program
                                                    #Question id: 23177
#Unit 13. Methods in Biology
(FRET) technique uses a pair of fluorescent proteins in which the emission wavelength of the first is 
 
                                                    