Phenyl ammonia lyase (PAL) involved in biosynthesis of phenolic compound in plants. Following are some statements regarding the action of PAL;

a) PAL catalyze conversion of phenyl alanine to p-coumaric acid

b) PAL catalyzes the conversion of phenyl alanine into trans-Cinnamic acid by elimination of an ammonia molecule

c) The activity of PAL is increased by environmental factors, such as low nutrient levels, light, and fungal infection

d) PAL is situated at a branch point between primary and secondary metabolism, so the reaction that it catalyzes is an important regulatory step in the formation of many phenolic compounds

Which of the following combinations of above statement is true?

TLS Online TPP Program

#Question id: 34170

#Unit 3. Fundamental Processes

What is correct about Klenow fragment

A. is a single polypeptide of 103 kD

B. larger cleavage product of DNA Pol I of 68 kD

C. It contains the polymerase activity

D. It contains the 3’-5’ exonuclease activity

E. It contains the 5’-3’ exonuclease activity

TLS Online TPP Program

#Question id: 34171

#Unit 3. Fundamental Processes

If the rate of fidelity of replication in bacteria is 10^-9/bp. How many replications it would take to make a mistake?

TLS Online TPP Program

#Question id: 34172

#Unit 3. Fundamental Processes

Frameshifts occur when an extra nucleotide is inserted or omitted. Which of sequence and property is most sensitive combination for the frameshift to occur

TLS Online TPP Program

#Question id: 34173

#Unit 3. Fundamental Processes

The 3’–5’ exonucleolytic activity is found in which subunit of bacterial DNA Polymerase III

TLS Online TPP Program

#Question id: 34174

#Unit 3. Fundamental Processes

Once bound to a primer:template junction, DNA polymerase interacts tightly with much of the double stranded portion of the DNA by

TLS Online TPP Program

#Question id: 34175

#Unit 3. Fundamental Processes

A. For processive DNA synthesis, proofreading occurs without releasing the DNA from the polymerase

B. When a mismatched base pair is present in the polymerase active site, the primer:template junction is destabilize

C. The DNA polymerase active site binds such a mismatched template poorly, but the exonuclease active site has a 10-fold higher affinity.

D. The incorrect nucleotide is removed by the 5’-3’-exonuclease nuclease activity

Choose true and false