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TLS Online TPP Program

#Question id: 10892

Considering environment and ecosystem, identify the INCORRECT statement

#Unit 10. Ecological Principles
  1. In detrital food chain, it is very difficult to measure the energy flow although the use of radioactive tracers give some idea about the energy flow
  2. The change in species composition, community structure and function over time is ecological succession
  3. The slower rise of environmental temperature may be attributed to the increase in pollution in environment which reflects enough solar energy back to other spacer to slow down global warming
  4. Photoperiodism has no relation with the environment and ecosystem, rather it is a biological event taking place in every plant
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TLS Online TPP Program

#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly
A- λ EMBL series                                                                                   I - 300kb - 1,400 kb
B - Cosmids                                                                                                 II - 45 kb
C – BACs and YACs (high capacity vectors)                                             III - 20kb
TLS Online TPP Program

#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program

#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 
TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 
TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop