A person’s normal tidal volume is 400 ml with a dead space of 100 ml. The respiratory rate is 12 breaths/min. The person is placed on ventilator for surgery and the tidal volume is 700 with a rate of 12. What is the approximate alveolar Pco2 for this person?

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#Question id: 18843

#Unit 13. Methods in Biology

Which of the following reagent is used for quantifying DNA

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#Question id: 18844

#Unit 13. Methods in Biology

In the extraction of DNA from cell with to remove the high protein content from the cell , before phenol treatment which of the following treatment is done 

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#Question id: 18845

#Unit 13. Methods in Biology

The action of EDTA in EDTA-lysozyme treatment in bacterial DNA extraction is

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#Question id: 18846

#Unit 13. Methods in Biology

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

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#Question id: 18847

#Unit 13. Methods in Biology

You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

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#Question id: 18848

#Unit 13. Methods in Biology

You find that your protein sample loses activity during storage. What can you do about this?