miRNAs are encoded in the genome as segments of longer transcripts. Their characteristic structure helps identify them and predict the target genes they might regulate. Which of the following are the characteristic features of these small RNA molecules?

I. The functional form of an miRNA is typically 21 or 22 nucleotides (it can vary from 19 to 25 nucleotides).

II. The first cleavage liberates the stem-loop, called the pre-miRNA; the second generates the mature miRNA from the pre-miRNA.

III. The pre-miRNAs can be encoded by any part of a transcript: that is, they might fall within coding regions, within leader regions, or within Introns.

IV. The base pairing between miRNA and target RNA is initiated by interactions of so-called seed residues—the sequence between bases 2 and 9 of the 22-nucleotide miRNA.

TLS Online TPP Program

#Question id: 19126

#Unit 12. Applied Biology

A successful biosensor must have some of the following features such as.

1. Should be highly specific for the analyte 

2. Reaction used should be independent of factors like stirring, pH, temperature, etc. 

3. Response should be linear over a useful range of analyte concentrations

Which of the following above statements are correct?

TLS Online TPP Program

#Question id: 32357

#Unit 1. Molecules and their Interaction Relevant to Biology

There are two classes of aldolases such as;

A) Class I aldolases B) Class II aldolases

i) Found in animals and plants

ii) Found in fungi and bacteria

iii) formation of protonated Schiff base on enzyme and its active site contain lysine residue

iv) do not form the Schiff base intermediate and a zinc ion at the active site is coordinated with the carbonyl oxygen at C-2

In which of the following option is the given enzyme with its correct statements?

TLS Online TPP Program

#Question id: 24257

#Unit 2. Cellular Organization

Choose the incorrect option

a. The nucleosome has an approximate twofold axis of symmetry, called the dyad axis

b. The nucleosome has an approximate tenfold axis of symmetry, divided in two axis

c. DNA exits at 1 o’clock and enters at just short of 11 o’clock on one axis and they change time after 180-degree rotation

d. DNA enters at 1 o’clock and exits at just short of 11 o’clock on one axis and they change time after 180-degree rotation

TLS Online TPP Program

#Question id: 28845

#Unit 2. Cellular Organization

The activity of the MAPs is regulated by the___

TLS Online TPP Program

#Question id: 19225

#Unit 12. Applied Biology

Modification of the tissue culture conditions by the inclusion of ________ during transformation of grape, rice, corn, or soybean has been found to increase the transformation frequencies of those plant cells.