Tyrosine contains a phenolic R-group and has pK1, pk2, pkR values of 2.20, 9.11, and 10.07 At which pH will all the tyrosine be in their fully protonated form?

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#Question id: 33698

#Unit 2. Cellular Organization

Globin gene clusters are found on chromosomes

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#Question id: 31234

#Unit 2. Cellular Organization

Failure of which surveillance mechanism is most likely to result in non-dysjunction of chromosomes?

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#Question id: 5584

#Unit 5. Developmental Biology

Which one of the following provides rapid block to polyspermy during sea urchin fertilization?

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#Question id: 18746

#Unit 13. Methods in Biology

Which of the following is a characteristic of Taq polymerase?

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#Question id: 13087

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?