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TLS Online TPP Program
#Question id: 18850
#Unit 13. Methods in Biology
Which of these chromatography types are suitable as a "capture" step in the purification of non-tagged proteins?
TLS Online TPP Program
#Question id: 18851
#Unit 13. Methods in Biology
Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?
TLS Online TPP Program
#Question id: 18852
#Unit 13. Methods in Biology
What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?
TLS Online TPP Program
#Question id: 18853
#Unit 13. Methods in Biology
Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?
TLS Online TPP Program
#Question id: 18854
#Unit 13. Methods in Biology
What properties of a protein does hydrophobic interaction chromatography exploit for purification?
TLS Online TPP Program
#Question id: 18855
#Unit 13. Methods in Biology
to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?