In response to stress, the adrenal gland promotes the synthesis of glucose from noncarbohydrate substrates via the action of the steroid hormone ________.

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#Question id: 2774

#Unit 2. Cellular Organization

The DNA and protein sequences of the -tubulin genes in humans and in fish are similar, and because each arose due to speciation, these genes would be considered:

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#Question id: 1133

#Unit 4. Cell Communication and Cell Signaling

What tertiary structure characterizes all cytokines?

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#Question id: 4953

#Unit 11. Evolution and Behavior

Several scientific laboratories across the globe are involved in research concerning the origin of life on Earth. Which graph below, if the results were produced abiotically, would have the greatest promise for revealing important information about the origin or Earthʹs first genetic system?

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#Question id: 19227

#Unit 12. Applied Biology

Genetically transformed plant using the A. tumefaciens strain expressing ACC deaminase, the transformation frequency of the plants

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#Question id: 13092

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?