#Question id: 4539
#Unit 3. Fundamental Processes
The yeast S. cerevisiae exists in three forms: two haploid cells of different mating types—a and alpha and the diploid formed when an a and an a cell mate and fuse. The a cell and the a cell each encodes cell-type-specific regulators: a cells make the regulatory protein a1, and alpha cells make the proteins alpha1 and alpha2. A fourth regulatory protein, called Mcm1, is also involved in regulating the mating-type-specific genes (and many other genes) and is present in both cell types which shows Combinatorial Control.
The arrangement of regulators at the promoters of a-specific genes and a-specific genes is
|
cell type: |
gene regulatory proteins: |
target genes: |
|
1.a cell (haploid) |
a1 Mcm1 |
aSG alphaSG |
|
2.αlpha cell (haploid) |
alpha 1 alpha 2 Mcm2 |
hSG |
|
3.a/αlpha cell (diploid) |
a1 Mcm2 alpha2 |
#Question id: 4540
#Unit 3. Fundamental Processes
Transcription of eukaryotic genes can be repressed in various ways. These include the four mechanisms
|
1. Competition |
a-Repression is caused by recruiting histone modifiers that alter nucleosomes in ways that inhibit transcript |
|
2. Inhibition |
b-A repressor binds to a site on DNA beside an activator and interacts with that activator, occluding its activating region |
|
3. Direct repression |
c-By binding to a site on DNA that overlaps the binding site of an activator, a repressor can inhibit binding of the activator to a gene and thus block activation of that gene. |
|
4. Indirect repression |
d-A repressor binds to a site upstream of a gene and, by interacting with the transcriptional machinery at the promoter in some specific way, inhibits transcription initiation. |
#Question id: 4541
#Unit 3. Fundamental Processes
Mig1 recruits a “repressing complex” containing the Tup1 protein. This complex is recruited by many yeast DNA-binding proteins that repress transcription, including the alpha2 protein involved in controlling the mating-type specific. Tup1 also has counterparts in mammalian cells, choose correct mechanisms have been proposed to explain the repressing effect of Tup1.
I-Tup1 acts on nucleosomes either through recruiting histone deacetylases and/or by positioning a nucleosome at or near the transcription start site.
II-Tup1 interacts directly with the transcriptional machinery at the promoter and inhibits initiation.
#Question id: 4542
#Unit 3. Fundamental Processes
Repression of the GAL1 gene in yeast by Mig1, in the presence of glucose follows those steps;
I- Deacetylation of local nucleosomes
II-Recruiting the Tup1 repressing complex
III- Mig1 represses expression of GAL1
IV- Mig1 binds a site between the UASG and the GAL1 promoter
#Question id: 4543
#Unit 3. Fundamental Processes
Control of the activator NtrC- In that case, the signal (low ammonia levels) induces a kinase that phosphorylates NtrC. This is type of
A. Indirect signaling
B. Direct signaling
C. Is an example of a signal transduction pathway
#Question id: 4544
#Unit 3. Fundamental Processes
How is the binding of ligand to the extracellular domain communicated to the intracellular domain?