Prion protein is a normal constituent of brain tissue in all mammals which of the following statements are correct regarding prion protein

a) Strains of mice lacking the gene for PrP suffer no obvious ill effect

b) Illness occurs only when the normal cellular PRP or prpc occurs in an altered confirmation called PrPsc

c) The interaction of prpsc with the prpc converts the letter to prpsc initiating a Domino effect in which more and more the brain proteins converted to the disease causing form

d) the structure of prpsc is very different with much of the structure converted to amyloid like Alpha sheet.

TLS Online TPP Program

#Question id: 18774

#Unit 13. Methods in Biology

The above Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. In these plots, r equals moles of bound ligand/mole antibody and c is the concentration of free ligand, (Ka) is slope while intercept on x axis represent 

TLS Online TPP Program

#Question id: 18775

#Unit 13. Methods in Biology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

TLS Online TPP Program

#Question id: 18776

#Unit 13. Methods in Biology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 

TLS Online TPP Program

#Question id: 18777

#Unit 13. Methods in Biology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

TLS Online TPP Program

#Question id: 18778

#Unit 13. Methods in Biology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

TLS Online TPP Program

#Question id: 18779

#Unit 13. Methods in Biology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true 

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response. 

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests