An experiment of Anfinsen on ribonuclease A (RNase A) showed that proteins can be denatured reversibly by 2-mercaptoethanol and 8 M urea. The protein must therefore renature spontaneously;

a) Remove mercaptoethanol and then removal of the urea

b) Removal of the mercaptoethanol allows the protein to re-form disulfide bonds in the presence of oxygen and then removal of the urea

c) Removal of the denaturant (urea) and reductant (mercaptoethanol) allows the protein to renature and re-form disulfide bonds in the absence of oxygen

d) Adding a small amount of mercaptoethanol to the scrambled protein in the absence of O2 catalyzes its conversion to the active enzyme

Which one of the following steps will leads to regaining of the full activity of ribonuclease A (RNase A)

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#Question id: 7562

#General Aptitude

A train does a journey without stopping in 8 hours. If it had traveled 5 km an hour faster, it would have done the journey in 6 hours 40 min. What is its slower speed?

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#Question id: 7563

#General Aptitude

Two trains travelling in the same direction at 40 kmph and 22 kmph completely pass each other in 1 minutes. If the length of first train is 125 m, what is the length of second train ?

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#Question id: 7564

#General Aptitude

A man by motorcycle goes from Delhi to Bharatpur , a distance of 192 km, at an average speed of 32 kmph. Another man starts from Delhi by car 2.5 h after the motorcyclist starts and reaches Bharatpur half an hour late. What is the ratio of person on the motorcycle to the person going by car ?

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#Question id: 7565

#General Aptitude

A Train T1 starts from Ahmedabad to Mumbai at 7 am and reaches at 12 noon. A second train T2 starts at 7 am from Mumbai reaches Ahmedabad at 1 pm. When did the two train cross each other ?

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#Question id: 7566

#General Aptitude

A car during its journey travels 30 minutes at a speed of 40 kmph, another 45 minutes at a speed of 60 kmph, and 2 hours at a speed of 70 kmph.The average speed of the car is

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#Question id: 7567

#General Aptitude

The Delhi Express runs @ 144 kilometers per hours and it reaches destination 20 minute ahead of schedule. If it averages 100 km/hour, it reaches 24 minute behind schedule. What is the average speed it should maintain in order to reach the destination on schedule?