An experiment of Anfinsen on ribonuclease A (RNase A) showed that proteins can be denatured reversibly by 2-mercaptoethanol and 8 M urea. The protein must therefore renature spontaneously;

a) Remove mercaptoethanol and then removal of the urea

b) Removal of the mercaptoethanol allows the protein to re-form disulfide bonds in the presence of oxygen and then removal of the urea

c) Removal of the denaturant (urea) and reductant (mercaptoethanol) allows the protein to renature and re-form disulfide bonds in the absence of oxygen

d) Adding a small amount of mercaptoethanol to the scrambled protein in the absence of O2 catalyzes its conversion to the active enzyme

Which one of the following steps will leads to regaining of the full activity of ribonuclease A (RNase A)

TLS Online TPP Program

#Question id: 31115

#Unit 2. Cellular Organization

Troponin C is closely related to the ubiquitous Ca2+-binding protein, called: 

TLS Online TPP Program

#Question id: 23831

#Unit 2. Cellular Organization

Which class of myosin is responsible for endocytosis and Membrane association?

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#Question id: 24597

#Unit 2. Cellular Organization

MAR fragments are usually

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#Question id: 33910

#Unit 2. Cellular Organization

What is the Lk of a 5,000 bp circular duplex DNA molecule after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP?

TLS Online TPP Program

#Question id: 28914

#Unit 4. Cell Communication and Cell Signaling

Wnt/calcium pathway leads to increased Ca²⁺ levels activates Ca²⁺-dependent signaling molecules such as; 

a) Protein kinase B

b) Protein kinase C

c) Ca²⁺/calmodulin-dependent protein kinase (CamKII),

d) Calcineurin phosphatase