Most proteins denature at both high and low pH. At high pH, the ionization of internal tyrosines is thought to be the main destabilizing influence, whereas at low pH, the protonation of buried histidines. A titration curve for the unfolding of the enzyme ribonuclease is shown in Figure. Given the relation between the titration curves for protein unfolding and histidine protonation, which of the following conclusion can be drawn correctly?

A. The sharp transition in graph indicates a highly cooperative process; when the protein starts to unfold, it completes the process rapidly

B. The sharp transition in graph indicates a highly noncooperative process

C. Several buried histidines can ionize when the chain starts to unfold, so that when one goes, they all go together due to cooperative behaviour of protein folding

D. Buried histidine (pK of 4 in this example) becomes accessible to solvent, its pK will shift toward its normal value of 6, significantly steepening its titration curve.

#Question id: 5648

#Unit 5. Developmental Biology

Use the following information to answer the question. In order to test how sea urchin sperm bind to eggs, scientists isolated the egg receptor protein that binds to the sperm acrosomal protein called bindin. Plastic beads were coated with egg receptor for bindin (ERB1) from eggs of the sea urchin Strongylocentrotus purpuratus, and then the beads mixed with sperm from S. purpuratus or from the related species, S. franciscanus. The researchers counted how many sperm were bound to each bead. The results are shown in the graph below. Treatments:

A: S. purpuratus sperm mixed with S. purpuratus ERB1 beads

B: S. purpuratus sperm mixed with beads containing no ERB1 protein

C: S. franciscanus sperm mixed with S. purpuratus ERB1 beads

D: S. franciscanus sperm mixed with beads containing no ERB1 protein

 What is a broader implication from the observations of the experiment?

#Question id: 5649

#Unit 5. Developmental Biology

From earliest to latest, the overall sequence of early development proceeds in which of the following sequences?

#Question id: 5650

#Unit 5. Developmental Biology

Thalidomide, now banned for use as a sedative during pregnancy, was used in the early 1960s by many women in their first trimester of pregnancy. Some of these women gave birth to children with limb and organ deformities, suggesting that the drug most likely influenced ________.

#Question id: 5651

#Unit 5. Developmental Biology

Growth and development of plant parts involves ________.

I) cell division to produce new cells

II) enlargement and elongation of cells

III) specialization of cells into tissues

#Question id: 5652

#Unit 5. Developmental Biology

What generalization can be applied to the pole plasm of Drosophila, the P-granules of C. elegans, the yolk-free vegetal cytoplasm of Xenopus, and the localized mRNA for vasa in zebrafish?

#Question id: 5653

#Unit 5. Developmental Biology

Why is meiosis required for germ cell formation, yet is never used by somatic cells?