#Question id: 5288
#Unit 8. Inheritance Biology
Donor strain of bacteria with His+ Ara+ Met+ Leu+ Cys+ Lys+ is infected with phages to map the donor chromosome with generalized transduction .The Phage lysate from bacterial cell is collected and use to infect second strain of bacteria that are His- Ara- Met- Leu- Cys- Lys- . Different transducing phages are selected on using different minimal media and their presence of growth (+) and absence of growth (-) given in table
Media |
Media supplement 0 absent /+ present |
||||||
1 |
His |
Ara |
Met |
Leu |
Cys |
Lys |
Growth |
2 |
+ |
+ |
0 |
+ |
+ |
0 |
+ |
3 |
0 |
+ |
+ |
+ |
+ |
0 |
+ |
4 |
+ |
+ |
0 |
+ |
0 |
+ |
- |
5 |
0 |
+ |
+ |
0 |
+ |
+ |
+ |
6 |
+ |
0 |
+ |
0 |
+ |
+ |
+ |
7 |
+ |
0 |
+ |
+ |
0 |
+ |
+ |
Which of the following is correct order of gene?
#Question id: 5289
#Unit 8. Inheritance Biology
A donor strain of bacteria with genes a+b+c+ is infected with phages to map the donor chromosome with transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a_ b_ c_.
Based on the above, which one of the following conclusions is correct?
#Question id: 5290
#Unit 8. Inheritance Biology
The following table shows mapping data from three interrupted mating experiments using three different Hfr strains and an F- strain: The order of genes is
#Question id: 5291
#Unit 8. Inheritance Biology
A number of nutritional mutant strains were isolated from wild-type Neurospora that responded to the addition of certain supplements in the culture medium by growth ( + ) or lack of growth ( -). Given the following responses for single gene mutations, diagram a metabolic pathway that could exist in the wild-type strain that is most consistent with the data. Indicate where the pathway is blocked in each mutant strain.
#Question id: 5292
#Unit 8. Inheritance Biology
A transformation experiment is performed with a donor strain that is resistant to four drugs: W, X, Y, and Z. The recipient is sensitive to all four drugs. The treated recipient population is divided up and plated on media containing various combinations of the drugs. The table below shows the results:
Drug(s) added |
Number of Colonies |
None |
20,000 |
WX |
76 |
WY |
1324 |
WZ |
1976 |
XY |
85 |
XZ |
69 |
YZ |
1564 |
WXY |
50 |
WXZ |
62 |
WYZ |
1354 |
XYZ |
48 |
WXYZ |
53 |
Following observation are given
A) X gene is obviously quite distant from the other three genes
B) Y gene is obviously quite distant from the other three genes
C) The Order is three closely link gene W Z Y
D) The Order is three closely link gene W X Z
Which of the following combination is correct?
#Question id: 5293
#Unit 8. Inheritance Biology
The two strains a and b are tested for being auxotrophic for the following nutritional mutations: pyridoxine (pdx), biotin (bio), methionine (met), adenine (ade), and para-aminobenzoic acid (pab). Growth is indicated by ( + ) and lack of growth by ( - ). From these tests, what is genotype of strain a and b respectively (+ indicate prototroph while other auxotroph)?