#Question id: 13076
#Unit 13. Methods in Biology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
#Question id: 18788
#Unit 13. Methods in Biology
#Question id: 24286
#Unit 4. Cell Communication and Cell Signaling
#Question id: 19874
#Unit 13. Methods in Biology
#Question id: 9589
#Unit 6. System Physiology – Plant
The physical mechanism by which excitation energy is conveyed from the chlorophyll that absorbs the light to the reaction centre, By this mechanism the excitation energy is transferred from one molecule to another by a nonradioactive process, this mechanism is known as