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TLS Online TPP Program
#Question id: 13089
#Unit 13. Methods in Biology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 2’s plasmid is;
TLS Online TPP Program
#Question id: 12980
#Unit 10. Ecological Principles
The following questions refer to Figure which depicts the age structure of three populations.
Which population is in the process of decreasing?
TLS Online TPP Program
#Question id: 2376
#Unit 2. Cellular Organization
Which organelle is composed of cristae and a matrix?
TLS Online TPP Program
#Question id: 18622
#Unit 13. Methods in Biology
Variants of PCR has been devised for mutational analysis
a) AS PCR
b) Site directed mutagenesis
c) Overlap extension PCR
d) Mega primer PCR
TLS Online TPP Program
#Question id: 15778
#Unit 5. Developmental Biology
Stem cell regulation is highly influenced by the microenvironment that surrounds a stem cell and is known as the