In this pedigree I 1 and II 2 have Becker muscular dystrophy which shows X-linked recessive inheritance. III 2 has oculocutaneous albinism which shows autosomal recessive inheritance with an incidence in the general population of 1 in 10 000. The disease status for both Becker muscular dystrophy and oculocutaneous albinism is unknown for IV 1 and IV 2.

What is the probability that IV 2 will be affected with Becker muscular dystrophy?

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#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?

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#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

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#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

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#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___

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#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I	COLUMN II
A) Enhance efficiency	i) Multiplex PCR
B) Sequence specific amplification	ii) Hot-start PCR
C) Mutational analysis	iii) Megaprimer PCR
	iv) qPCR with SYBR
	v) AS-PCR
	vi) RLM-RACE

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#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?