In Neurospora crasssa, the mutant exhibit poky phenotype, when a female of  poky strain is crossed with a normal strain acting as a male , all progeny individuals shows poky phenotype . however, the reciprocal cross resulted in all normal progeny. These results can be explained on the basis of 

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#Question id: 17659

#Unit 13. Methods in Biology

During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 

a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.

b) -1 frameshift is inserted after the amber so that no longer read as a stop.

c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.

d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.

Which of the following is the correct prediction about the frame shift mutation?

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#Question id: 17819

#Unit 13. Methods in Biology

During RNA extraction, the homogenate is first treated with

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#Question id: 17820

#Unit 13. Methods in Biology

During the RNA extraction guanidinium thiocyanate functions as a strong inhibitor of

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#Question id: 17997

#Unit 13. Methods in Biology

In RNA isolation, for protein free solution what will be the ratio at  nm

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#Question id: 17998

#Unit 13. Methods in Biology

Eukaryotic mRNA species are isolated by using

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#Question id: 18074

#Unit 13. Methods in Biology

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use