According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 18624

#Unit 13. Methods in Biology

In GFP protein oxidation of tripeptide sequence gives fluorescence the tripeptide sequence made up of

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#Question id: 1965

#Unit 4. Cell Communication and Cell Signaling

In some insects, such as Drosophila, fungal cell wall elements can activate the protein Toll. What is Tollʹs function?

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#Question id: 26427

#Unit 3. Fundamental Processes

Which of the following component recognizes single-base mismatches

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#Question id: 19696

#Unit 13. Methods in Biology

Different leads are used to record ECG of humans. Which of the following connection shows in recording limb lead I?

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#Question id: 2019

#Unit 4. Cell Communication and Cell Signaling

Which of the following is not a component of an insectʹs defense against infection?