Why mRNA cannot be used directly in cloning?

TLS Online TPP Program

#Question id: 1377

#Unit 4. Cell Communication and Cell Signaling

Which of the following statements apply to both eukaryotic cell walls and animal cell extracellular matrices? Please select all that apply.

a) They are inflexible structures that support cells.

b) They are built to a common overall structure that can be described as 'fibres in a matrix'.

c) Their protein components are synthesized by ribosomes of the rough endoplasmic reticulum.

d) They limit the diffusion of ions and small molecules into the cell.

TLS Online TPP Program

#Question id: 5555

#Unit 5. Developmental Biology

The formation of the fertilization envelope requires an increase in the cytosolic concentration of ________.

TLS Online TPP Program

#Question id: 15681

#Unit 2. Cellular Organization

 Moist heat kills microorganisms by

TLS Online TPP Program

#Question id: 4205

#Unit 3. Fundamental Processes

Puromycin is an antibiotic that inhibits protein synthesis because

TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop