Which the following statements is/are true about the stacking gel?

i. The stacking gel solution contains acrylamide.

ii. It is on the top of the separating gel.

iii. It is used to dilute the protein sample before it enters the main separating gel.

iv. It is into this gel that the wells are formed and the proteins loaded.

#Question id: 10954

#Unit 7. System Physiology – Animal

A patient suffers from a congenital deficiency in factor XIII (fibrin-stabilizing factor). What would analysis of his blood reveal?

#Question id: 4284

#Unit 3. Fundamental Processes

Assays for following the trafficking of proteins through the secretory pathway in living cells require

a. sensitive methods to distinguish the very small differences in organelle density caused by passage of a wave of protein transport through the organelle.

b. a way to identify compartments where labeled proteins are located.

c. the use of cell types that are highly dedicated secretory cells.

d. the use of enucleate cells in order to eliminate the nucleus as a site of labeling.

e. a way to label a cohort of secretory proteins

#Question id: 10533

#Unit 2. Cellular Organization

Following statements are regarding to the fluidity of a bilayer.

A. A lipid bilayer cools below a characteristic transition temperature, it undergoes a sort of phase change in which it becomes a liquid; that is, it loses its fluidity.

B. Above the transition temperature, the highly mobile lipids are in a state known as a liquid crystal.

C. The bilayer is thicker in the gel state than in the liquid crystal state due to the stiffening of the hydrocarbon tails at lower temperatures.

D. The transition temperature of a bilayer decreases with the chain length and the degree of saturation of its component fatty acid residues for the same reasons that the melting points of fatty acids increase with these quantities.

Which of the following combination is correct?

#Question id: 11920

#Unit 10. Ecological Principles

Erigeron dominates and reduces the biomass of Setaria, this is an example of

#Question id: 3818

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were semi-conservative, what results would be found when the double-stranded DNA was analyzed in a CsCl density gradient after three generations in 15N-medium?