Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

TLS Online TPP Program

#Question id: 32133

#Unit 1. Structure and Function of Biomolecules

The structure shown here is 

TLS Online TPP Program

#Question id: 32134

#Unit 1. Structure and Function of Biomolecules

In mammals, which nucleotide act as local hormone, and as a neuromodulator

TLS Online TPP Program

#Question id: 32135

#Unit 1. Structure and Function of Biomolecules

The net charge on a nucleoside monophosphate is 

TLS Online TPP Program

#Question id: 32137

#Unit 1. Structure and Function of Biomolecules

The degrees of freedom in the sugar–PO4 backbone of DNA chain is/are

TLS Online TPP Program

#Question id: 32138

#Unit 1. Structure and Function of Biomolecules

The bond angle of glycosidic bond is

TLS Online TPP Program

#Question id: 32139

#Unit 1. Structure and Function of Biomolecules

The core of the helix consists of the base pairs that stack together through