Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 18908

#Unit 13. Methods in Biology

pBR 322 has/have which of the following selection marker(s)?

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#Question id: 18909

#Unit 13. Methods in Biology

What is the compound X-gal?

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#Question id: 18910

#Unit 13. Methods in Biology

is a lactose analog which is broken down by enzyme beta-galactosidase to give a product deep blue in colour.

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#Question id: 18911

#Unit 13. Methods in Biology

All vectors used for propagation of DNA inserts in a suitable host are called………….. But when a vector is designed for the expression of, i.e., production of the protein specified by, the DNA insert, it is termed as ………..

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#Question id: 18912

#Unit 13. Methods in Biology

In PBR322,n in the AmpR there are restriction site are for PstI,  while tetracycline resistance have restriction site are for

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#Question id: 18913

#Unit 13. Methods in Biology

In PBR322, If a DNA with PstI will be selected on the basis of