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TLS Online TPP Program

#Question id: 13054

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Unit 13. Methods in Biology
  1. The PCR reaction would not commence
  2. The PCR reaction would end after one cycle
  3. The reaction would generate a single short PCR product
  4. The reaction would yield a mixture of non-specific products
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TLS Online TPP Program

#Question id: 2281

#Unit 2. Cellular Organization

Enzymes embedded in the membrane of the smooth ER
TLS Online TPP Program

#Question id: 24285

#Unit 4. Cell Communication and Cell Signaling

Adherens junctions: 
TLS Online TPP Program

#Question id: 872

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following statements is not true about HSP 70 family.

A. They have a molecular weight near 70,000 and are abundant in cells stressed by elevated temperature.

B. Bind to regions of unfolded polypeptides that are rich in hydrophilic residues

C. Hsp70 proteins not  block the folding of certain proteins that must remain unfolded untill they have been translocated across a membrane.

D.) It releases polypeptides in a cycle that uses energy from ATP hydrolysis.
TLS Online TPP Program

#Question id: 12792

#Unit 10. Ecological Principles

Many  homeowners mow  their  lawns  during the  summer and  collect  the  clippings,  which  are  then  hauled to  the local landfill. Which of the following actions would most benefit the local ecosystem?
TLS Online TPP Program

#Question id: 24619

#Unit 2. Cellular Organization

Which of the following function occupies most genome part for activity in eukaryotic genome