Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 20293

#Unit 7. System Physiology – Animal

Other than CRH, what can stimulate the secretion of ACTH?

TLS Online TPP Program

#Question id: 20294

#Unit 7. System Physiology – Animal

The rate-limiting step during the synthesis of all steroid hormones is done by which enzyme? 

TLS Online TPP Program

#Question id: 20295

#Unit 7. System Physiology – Animal

Catecholamines can lead to hyperglycemia due to: 

TLS Online TPP Program

#Question id: 20296

#Unit 7. System Physiology – Animal

Aldosterone acts on: 

TLS Online TPP Program

#Question id: 20297

#Unit 7. System Physiology – Animal

Hyperpigmentation is a sign of: 

TLS Online TPP Program

#Question id: 20298

#Unit 7. System Physiology – Animal

What can stimulate aldosterone secretion: