Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

#Question id: 19108

#Unit 8. Inheritance Biology

#Question id: 19043

#Unit 13. Methods in Biology

#Question id: 3831

#Unit 3. Fundamental Processes

State whether the following statements is true/false regarding DNA replication.

1. In E. coli replication begins at the origin of replication and proceeds in one direction until the entire circular DNA molecule has been copied.

2. The chromosome of the fruit fly (D. melanogaster) is about twice as large as the E. coli chromosome.

3. Prokaryotic DNA replication occurs in two steps. First, ATP provides a phosphate to the growing DNA chain. This is followed by addition of a nucleoside.

4. DNA polymerase III is the largest DNA polymerase in E. coli.

5. The β subunits of E. coli DNA polymerase form a sliding clamp that surrounds the DNA strands at the replication fork.

#Question id: 1096

#Unit 4. Cell Communication and Cell Signaling

Which of the following processes generally requires protein phosphorylation?

#Question id: 1210

#Unit 4. Cell Communication and Cell Signaling

Proteins with SH2 domains are to ...........as adenylyl cyclase and phospholipase C are to G protein-coupled receptors.