Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

TLS Online TPP Program

#Question id: 26469

#Unit 3. Fundamental Processes

RNA proofreading and backtrack in the prokaryotes is done by

TLS Online TPP Program

#Question id: 26470

#Unit 3. Fundamental Processes

Choose which of the following statement is incorrect regarding the TCRF

TLS Online TPP Program

#Question id: 26471

#Unit 3. Fundamental Processes

Intrinsic terminators are responsible for prokaryotic transcription termination, so which of the following is the characteristics of Intrinsic terminators

a. It  include palindromic regions 

b. It do not include palindromic regions 

c. Palindromic regions that form hairpins varying in length from 7 to 20 bp

d. The stem-loop structure includes a G-C–rich region and is followed by a run of 7U residues.

TLS Online TPP Program

#Question id: 26472

#Unit 3. Fundamental Processes

The script that do not have intrinsic terminators known as

TLS Online TPP Program

#Question id: 26473

#Unit 3. Fundamental Processes

rut sites are

TLS Online TPP Program

#Question id: 26474

#Unit 3. Fundamental Processes

Choose the incorrect option regarding Rho protein