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TLS Online TPP Program

#Question id: 13056

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
 Which of the following is true for traditional and real time PCR?

#Unit 13. Methods in Biology
  1. Both the techniques allow detection of the product in early stages
  2. Real time PCR can detect the product in early stages as compared to traditional PCR
  3. Both systems require different primers
  4. Both techniques are end point product determination methods

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TLS Online TPP Program

#Question id: 2580

#Unit 2. Cellular Organization

Following statements are regarding to mechanism of kinesin-1 movement.

A. Initially leading head tightly bound to the microtubule and not bound by any nucleotide, while the trailing head is weakly bound to the microtubule and has ADP bound.

B. Binding of ATP induces a conformational change causing the linker to swing forward and dock into the head. This motion swings the former trailing head to become the leading head.

C. Binding of ATP induces a conformational change causing the linker to swing forward and dock into the head. This motion swings the former leading head to become the trailing head.

D. When leading head releases ADP and coordinately the trailing head hydrolyzes ATP to ADP + Pi. Pi is released and the linker becomes undocked.

E. When trailing head releases ADP and coordinately the leading head hydrolyzes ATP to ADP + Pi. Pi is released and the linker becomes undocked.

Which of the following combination is correct?
TLS Online TPP Program

#Question id: 15849

#Unit 4. Cell Communication and Cell Signaling

Host-pathogen interactions are an ongoing arms race; pathogens evolve to express unique structures that avoid host detection, and the host germline-encoded recognition system co-evolves to match these new challenges. However, because pathogens generally have much shorter life cycles than their vertebrate hosts, and some utilize error-prone DNA polymerases to replicate their genomes, pathogens can evolve rapidly to evade host encoded recognition systems. Which error prone DNA polymerase is used  by human?
TLS Online TPP Program

#Question id: 464

#Unit 1. Molecules and their Interaction Relevant to Biology

Calculate the equilibrium constant for an oxidation-reduction reaction involving the transfer of 1 mole of electrons with DE0ʹ= 0.25 V. (R = 8.315 JK-1mol-1, F = 96.48 kJV-1mol-1, T = 298 K)
TLS Online TPP Program

#Question id: 3870

#Unit 3. Fundamental Processes

“Footprinting” or DNase protection is a technique used to identify:
TLS Online TPP Program

#Question id: 27014

#Unit 10. Ecological Principles

competition can be observed between plants, where one species produces & secrete chemicals & inhibits the growth of another species, is an example of