Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 10771

#Unit 8. Inheritance Biology

What is penetrance?

A- % of individuals having phenotype according to their genotype.

B- Penetrance refers to the likelihood that a clinical condition will occur when a particular genotype is absent.

C- 100% expressivity always gives 100% penetrance.

which one of the correct combination-

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#Question id: 10772

#Unit 8. Inheritance Biology

In an experiment plant RR round genotype have 100 seeds. In F1 generation 70 seeds are round and 30 seeds are wrinkled. So what will be

the penetrance value for round seeds?

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#Question id: 10773

#Unit 8. Inheritance Biology

What is Pseudo dominance?

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#Question id: 11617

#Unit 8. Inheritance Biology

Individuals with a particular genotype that express the phenotype expected of that genotype, this is known as

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#Question id: 11618

#Unit 8. Inheritance Biology

A monohybrid cross between two heterozygous  yellow mice forms the progeny in F1 generation  is  yellow and  non-yellow with the genotype YY(Lethal) and yy , so what will be the ratio of this cross?

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#Question id: 11619

#Unit 8. Inheritance Biology

If a cross between restricted and dusky  ducks  the only progeny will be form from this cross is restricted and mallard, so what will be the genotype of parents?