Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 11986

#Unit 10. Ecological Principles

Which of the following statements is (are) true of fixed action patterns? 

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#Question id: 15992

#Unit 12. Applied Biology

The genetic variability present among cultured cells, progeny of such plants is called__

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#Question id: 1510

#Unit 4. Cell Communication and Cell Signaling

Match the correct protein (Column I) with their function (Column II) during apoptosis.

Column I	Column II
A. Puma	I. Pro-survival protein
B. Bak	II. Zymogen
C. Mcl-1	III. Pro-apoptotic protein
D. CED-3	IV. BH3-only protein

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#Question id: 4001

#Unit 3. Fundamental Processes

Which is not true about the lac repressor?

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#Question id: 12618

#Unit 10. Ecological Principles

Exponential growth of a population is represented by dN/dt