Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 23127

#Unit 2. Cellular Organization

Which of the following can be used to test same gene in different organism

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#Question id: 23128

#Unit 2. Cellular Organization

When pairs of human 23 and mouse 20 homologs are compared, the genes located on either side also tend to be homologs so this homology can be compared by using

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#Question id: 23129

#Unit 2. Cellular Organization

Which of the following Hypothesis states that introns have always been an integral part of the gene.

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#Question id: 23130

#Unit 2. Cellular Organization

Which of the following structural component differentiate between pathogenic and nonpathogenic variants of the same or related species

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#Question id: 23131

#Unit 2. Cellular Organization

How the minimum no. of proteome can be estimated

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#Question id: 23132

#Unit 2. Cellular Organization

Which of the following component has the highest proportion in human genome