Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 31405

#Unit 5. Developmental Biology

The acquisition of a given cell identity based on its position or, more specifically, on the interactions that cell has with the other cells, phenomena known as:

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#Question id: 31408

#Unit 5. Developmental Biology

The Drosophila Sex-lethal (Sxl) gene is activated in females, but the Sxl protein does not form in males because of: 

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#Question id: 9268

#Unit 6. System Physiology – Plant

The most active photosynthetic tissue in higher plants is the

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#Question id: 9269

#Unit 6. System Physiology – Plant

The distribution of electrons in the excited molecule is somewhat different from the distribution in the groundstate molecule, Absorption of blue light excites the chlorophyll to a higher energy state than absorption of red light because

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#Question id: 9270

#Unit 6. System Physiology – Plant

In the lowest excited state, the excited chlorophyll has four alternative pathways for disposing of its available energy which of the statement of alternative pathway would be INCORRECT?

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#Question id: 9271

#Unit 6. System Physiology – Plant

Chlorophylls a and b are abundant in green plants, and c and d are found in some protists and cyanobacteria. A number of different types of bacteriochlorophyll have been found; which type of chlorophyll is the most widely distributed, shows the distribution of pigments in different types of photosynthetic organisms;