Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 23158

#Unit 13. Methods in Biology

You have purified protein X and injected into an animal so that over a period of weeks, the animal will mount an immune response and make antibodies to protein X. By which technique these generated antibodies can be extracted from blood serum.

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#Question id: 23159

#Unit 13. Methods in Biology

Rhodamine is a fluorescent dye which emits a colour?

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#Question id: 23160

#Unit 13. Methods in Biology

Texas is a fluorescent dye which emits a colour?

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#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?

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#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?

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#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?