Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

#Question id: 12636

#Unit 6. System Physiology – Plant

#Question id: 28395

#Unit 5. Developmental Biology

One of the genes activated by VegT protein encodes the Sox17 transcription factor , these sox17 transcription factor is critical for which functions?

#Question id: 5528

#Unit 5. Developmental Biology

The lining of the uterus in which the mammalian embryo implantationsTakes place called as

#Question id: 27559

#Unit 1. Molecules and their Interaction Relevant to Biology

Since the pK values for aspartic acid are 2.0, 3.9 and 10.0, it follows that the isoelectric (pH) is

#Question id: 9227

#Unit 9. Diversity of Life Forms

A land developer and several ecologists are discussing how a parcel of private land should be developed with housing while saving 20 hectares as natural habitat. The land developer suggests that the 20 hectares be divided into 20 separate 1-hectare areas, spread out across the area. The ecologists suggest that it would be better to have one intact parcel of 20 hectares on one side of the larger area. What is the significance of these different arrangements of the 20 hectares?