Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 23794

#Unit 7. System Physiology – Animal

The responses of amacrine cells produce 

TLS Online TPP Program

#Question id: 23795

#Unit 7. System Physiology – Animal

Correctly match the responses with given cells according their functions;

Cells	Responses
1.Rods, cones and horizontal cells	p. hyperpolarizing potential
2.Bipolar cells	q. depolarizing potential
3.Amacrine cells	r. Either hyperpolarizing or depolarizing potential

TLS Online TPP Program

#Question id: 24453

#Unit 7. System Physiology – Animal

A visual exam in an 80-year-old man shows he has a reduced ability to see objects in the upper and lower quadrants of the left visual fields of both eyes but some vision remains in the central regions of the visual field. The diagnosis is

TLS Online TPP Program

#Question id: 24454

#Unit 7. System Physiology – Animal

A 45-year-old female who had never needed to wear glasses experienced difficulty reading a menu in a dimly-lit restaurant. She then recalled that as of late she needed to have the newspaper closer to her eyes in order to read it. A friend recommended she purchase reading glasses. Visual accommodation involves

TLS Online TPP Program

#Question id: 24455

#Unit 7. System Physiology – Animal

A 28-year-old male with severe myopia made an appointment to see his ophthalmologist when he began to notice flashing lights and floaters in his visual fi eld. He was diagnosed with a retinal detachment. The retina

TLS Online TPP Program

#Question id: 24456

#Unit 7. System Physiology – Animal

A 62-year-old Caucasian woman experienced a rapid onset of blurry vision along with loss of central vision. A comprehensive eye exam showed that she had wet age-related macular degeneration. The fovea of the eye