Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 2419

#Unit 2. Cellular Organization

During treadmilling, actin subunits add:

TLS Online TPP Program

#Question id: 2420

#Unit 2. Cellular Organization

Which of the following proteins promotes actin assembly and is involved in signaling pathways controlling actin assembly at the plasma membrane?

TLS Online TPP Program

#Question id: 2421

#Unit 2. Cellular Organization

Listeria is a bacterial parasite that has evolved unique ways to enter animal cells and then use actin polymerization for its intracellular movement.  Which of the following statements is true in regard to Listeria and actin polymerization?

TLS Online TPP Program

#Question id: 2422

#Unit 2. Cellular Organization

 What actin-binding protein mediates gel-sol transitions?

TLS Online TPP Program

#Question id: 2423

#Unit 2. Cellular Organization

What is the function of thymosin beta-4?

TLS Online TPP Program

#Question id: 2424

#Unit 2. Cellular Organization

Which of the following statements correctly describe(s) actin?

a. Actin is a highly conserved protein.

b. Multiple actin genes may be found in higher eukaryotes.

c. Actin is the most abundant protein in a eukaryotic cell.

d. Individual actin filaments can be observed by electron microscopy.

e. One end of an actin filament is different from the other.