Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

TLS Online TPP Program

#Question id: 28699

#Unit 2. Cellular Organization

First sugar residue is attached to dolichol by ___

TLS Online TPP Program

#Question id: 28981

#Unit 7. System Physiology – Animal

Phenylketonuria (PKU) is caused by___

I-Mutation of the gene for phenylalanine hydroxylase

II-tetrahydrobiopterin (BH4) deficiency

III-mutation in dihydroxyphenylalanine 

IV-due to the substitution mutation in tetrahydrobiopterin

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#Question id: 10948

#Unit 7. System Physiology – Animal

Fluid exudation into the tissue in the acute inflammatory reaction is due to

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#Question id: 33416

#Unit 1. Molecules and their Interaction Relevant to Biology

GrpE, catalyzes 

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#Question id: 10202

#Unit 6. System Physiology – Plant

What will happen if C4 leaves are exposed to low O2 concentrations?