Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

#Question id: 10131

#Unit 6. System Physiology – Plant

The detailed chemical mechanism of the oxidation of water to O2 is not yet well understood, but with structural information shows_one electron carrier, generally identified as Yz, functions between the oxygen-evolving complex and P680 to function in this region, Yz needs to have a very strong tendency to retain its electrons, this species has been identified as;

#Question id: 10132

#Unit 6. System Physiology – Plant

Which of the following statements would be correct for photophosphorylation ?

#Question id: 10133

#Unit 6. System Physiology – Plant

The PSI reaction center complex is a large multi-subunit complex in which the antenna chlorophylls are associated with the reaction center Components of the PSI reaction center are organized around as follows choose the incorrect one;

#Question id: 10134

#Unit 6. System Physiology – Plant

Some herbicides such as dichlorophenyldimethylurea (DCMU, also known as diuron) and paraquat, block photosynthetic electron flow from where ?

a.) DCMU blocks electron flow at the quinone acceptors of PSII, by competing for the binding site of plastoquinone that is normally occupied by PQB

b.) Paraquat accepts electrons from the early acceptors of PSI and then reacts with oxygen to form superoxide, O2 – , a species that is very damaging to chloroplast components

c.) DCMU blocks electron flow at the pheophytin acceptors of PSII, by competing for the binding site of pheophytin

d.) Paraquat accepts electrons from the Iron-sulfur system of PSI and then reacts with oxygen to form superoxide, O2 – , a species that is very damaging to chloroplast components

Which of the following combination is true?

#Question id: 10135

#Unit 6. System Physiology – Plant

Which of the following are strongest reductant?

#Question id: 10136

#Unit 6. System Physiology – Plant

A protein complex contain a Rieske protein known as