Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

#Question id: 1530

#Unit 4. Cell Communication and Cell Signaling

What are the differences and similarities between the transforming genes of retroviruses and those of DNA tumor viruses?

#Question id: 1532

#Unit 4. Cell Communication and Cell Signaling

For a particular microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumor biopsies. Which of the following types of evidence will researchers be looking for in order to determine if the cells are cancerous?

#Question id: 1533

#Unit 4. Cell Communication and Cell Signaling

Why might using retroviral vectors for gene therapy increase the patient's risk of developing cancer?

#Question id: 1534

#Unit 4. Cell Communication and Cell Signaling

Cadherin proteins help animal cells stick (adhere) to each other. Choose which statement about cadherin in cancer cells that are metastasizing (spreading) throughout a patient's body is most likely correct.

#Question id: 1535

#Unit 4. Cell Communication and Cell Signaling

Following statements are regarding to cancer stem cell (CSCs).

a. A representative subpopulation of cancer cells with peculiar molecular aspects that resemble some of those typical of normal stem cells.

b. These cells are capable of replenishing the repertoire of identical cancer cells.

c. These cells cannot create heterogeneous progeny that differentiate into more mature cells.

d. These cells show extraordinary proliferative potential.

e. These cells show excessive programmed cell death.

Which of the following combination is correct?

#Question id: 1536

#Unit 4. Cell Communication and Cell Signaling

Use the following figure to answer the question. According to figure, which of the following statement is incorrect?