Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 608

#Unit 1. Molecules and their Interaction Relevant to Biology

The ʺTʺ state refers to the ________.

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#Question id: 11374

#Unit 9. Diversity of Life Forms

Which of the following use light energy to generate ATP, but do not release oxygen?

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#Question id: 19464

#Unit 13. Methods in Biology

Which is the common fixative agent that crosslinks amino groups on adjacent molecules

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#Question id: 12025

#Unit 7. System Physiology – Animal

After surgical removal of an infected gallbladder, a person must be especially careful to restrict dietary intake of ________.

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#Question id: 6597

#General Aptitude

Z	?	S
R	O	?
?	G	C