Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

#Question id: 27945

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following statements is true regarding the payoff phase of glycolysis

a. Each molecule of glyceraldehyde 3-phosphate is oxidized and phosphorylated by ATP to form 1,3-bisphosphoglycerate 

b. Each molecule of glyceraldehyde 3-phosphate is oxidized and phosphorylated by inorganic phosphate to form 1,3-bisphosphoglycerate

c. Oxidative phosphorylation of 3-Phosphoglycerate produces one NADH

d. Oxidative phosphorylation of glyceraldehyde 3-phosphate, produces one NADH

#Question id: 27946

#Unit 1. Molecules and their Interaction Relevant to Biology

#Question id: 27947

#Unit 1. Molecules and their Interaction Relevant to Biology

For each molecule of glucose degraded to pyruvate, two molecules of ATP are generated from ADP and Pi, and two molecules of NADH are produced by the reduction of NAD+. The hydrogen acceptor in this reaction is NAD+  this NAD+ bound to a

#Question id: 27948

#Unit 1. Molecules and their Interaction Relevant to Biology

#Question id: 27949

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following reaction is catalyzed by enzyme phosphohexose isomerase (phosphoglucose isomerase)

#Question id: 27950

#Unit 1. Molecules and their Interaction Relevant to Biology

Some of the properties of Hexokinases I, II, III & IV is given

a. Hexokinases I, II, and III shows hyperbolic curve of glucose binding while Hexokinase IV shows sigmoidal curve 

b. Accumulation of glucose 6-phosphate inhibits hexokinases I–III by feedback mechanism 

c. Accumulation of glucose 6-phosphate allosterically inhibits hexokinases I–III.

d. The allosteric effector of Hexokinase IV is fructose 6-phosphate.

Which of the following is incorrect