Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 395

#Unit 1. Molecules and their Interaction Relevant to Biology

How would acidification of seawater affect marine organisms? Acidification of seawater would ________.

#Question id: 396

#Unit 1. Molecules and their Interaction Relevant to Biology

Identical heat lamps are arranged to shine on two identical containers, one containing water and one methanol (wood alcohol), so that each liquid absorbs the same amount of energy minute by minute. The covalent bonds of methanol molecules are nonpolar, so there are no hydrogen bonds among methanol molecules. Which of the following graphs correctly describes what will happen to the temperature of the water and the methanol?

#Question id: 397

#Unit 1. Molecules and their Interaction Relevant to Biology

The loss of water from a plant by transpiration cools the leaf. Movement of water in transpiration requires both adhesion to the conducting walls and wood fibers of the plant and cohesion of the molecules to each other. A scientist wanted to increase the rate of transpiration of a crop species to extend its range into warmer climates. The scientist substituted a nonpolar solution with an atomic mass similar to that of water for hydrating the plants. What do you expect the scientist's data will indicate from this experiment?

#Question id: 398

#Unit 1. Molecules and their Interaction Relevant to Biology

One of the buffers that contribute to pH stability in human blood is carbonic acid (H2CO3). Carbonic acid is a weak acid that, when placed in an aqueous solution, dissociates into a bicarbonate ion (HCO3-) and a hydrogen ion (H+). (See figure.)

If the pH of blood drops, one would expect ________.

#Question id: 399

#Unit 1. Molecules and their Interaction Relevant to Biology

Calculate the pH of a 1 L solution containing 0.100 M formic acid and 0.100 M sodium formate before and after the addition of 1.00 mL of 5.00 M NaOH. How much would the pH change if the NaOH were added to 1.00 L of pure water? (pKa for formic acid is 3.75)

#Question id: 400

#Unit 1. Molecules and their Interaction Relevant to Biology

Consider two solutions: solution X has a pH of 4; solution Y has a pH of 7. From this information, we can reasonably conclude that ________.