Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 28621

#Unit 2. Cellular Organization

Substrate-targeting subunits of the APC/C recognize specific sequence motifs on the proteins that the APC/C ubiquitinates. One motif the destruction box or D-box, has the consensus sequence is___

TLS Online TPP Program

#Question id: 28622

#Unit 2. Cellular Organization

What will cause if mutating or deleting the destruction box in mitotic cyclins? 

a) Allows loading of MCM helicases

b) prevented mitotic cyclins ubiquitinylation

c) blocked mitotic cells from exiting mitosis

d) inhibition of re-initiation at origins

TLS Online TPP Program

#Question id: 28623

#Unit 2. Cellular Organization

At which phase of APC/C bound to Cdc20 targets protein for degradation? 

TLS Online TPP Program

#Question id: 28624

#Unit 2. Cellular Organization

Ubiquitin protein-ligases APC/CCdc20  performs various functions such as  

a) Degradation of geminin

b) Degradation of securin

c) Induce degradation of B-type cyclin

d) Allows chromosomal separation

Which of the following is incorrect about APC/CCdc20

TLS Online TPP Program

#Question id: 28625

#Unit 2. Cellular Organization

Ubiquitin protein-ligases APC/CCdh1  performs various functions such as  

a) Degradation of geminin

b) Degradation of securin

c) Induce degradation of B-type cyclin in G1

d) Allows loading of  replicative helicase 

Which of the following is incorrect about APC/CCdh1

TLS Online TPP Program

#Question id: 28626

#Unit 2. Cellular Organization

At which phase of APC/C bound to Cdh1 targets protein for degradation?