Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

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#Question id: 28943

#Unit 7. System Physiology – Animal

Hypothalamus secreting Corticotropin-releasing hormone (CRH) stimulates the secretion of____

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#Question id: 28944

#Unit 7. System Physiology – Animal

Which of the following Prediction is correct about adrenocorticotropic hormone (ACTH) ? 

a) CRH Stimulates secretion of ACTH by corticotropes

b) it controls the secretion of cortisol by the activation of adenylyl cyclase via Gαq

c) it controls the secretion of cortisol by the activation of adenylyl cyclase via Gαs

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#Question id: 28945

#Unit 7. System Physiology – Animal

Which of the following hormones is involved to promotes the mammary gland development and milk production?

TLS Online TPP Program

#Question id: 28946

#Unit 7. System Physiology – Animal

GnRH is carried to the anterior pituitary, stimulates the secretion of hormones by gonadotropes. these hormones are; 

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#Question id: 28947

#Unit 7. System Physiology – Animal

Kallmann syndrome exhibit gonadal dysfunctions in male, which of the following statements is incorrect ?

A. a condition that causes hypogonadotropic hypogonadism (HH) 

B. an impaired sense of smell called anosmia 

C. it causes due to the deficiency of gonadotropin-releasing hormone (GnRH) 

D. they have higher level of circulating gonadotropins

E. a condition that causes hypergonadotropic hypergonadism (HH)

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#Question id: 28948

#Unit 7. System Physiology – Animal

FSH and LH is involved to which of the following activity?