To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 11802

#Unit 6. System Physiology – Plant

Damaged sieve elements are sealed off, but because of the processs that plug sieve plates pores, excuded phloem sap from wounds that sever sieve elements. Exudation of sap enhanced by the inclusion of;

#Question id: 11803

#Unit 6. System Physiology – Plant

Due to damage exudation of sap from cut petioles or stems takes place they can be collected. However, quantities of collected sap are small, and the method is technically difficult. A preferable approach for collecting exuded sap is to use an

#Question id: 11804

#Unit 6. System Physiology – Plant

P-proteins have been characterized at the molecular level. P-proteins from the genus Cucurbita consist of two major proteins;

#Question id: 11805

#Unit 6. System Physiology – Plant

P-proteins from the genus Cucurbita consist of two major proteins: PP1, the phloem filament protein, and PP2, the phloem lectin. Both PP1 and PP2 are thought to be synthesized in_______A___________and transported via the_______B_________to the_______C______, where they associate to form P-protein filaments and P-protein bodies.

#Question id: 11806

#Unit 6. System Physiology – Plant

sap is translocated from areas of supply, called sources, to areas of metabolism or storage, called sinks. Which of the following statements about source and sink are TRUE?

a.) Sinks include any nonphotosynthetic organs of the plant and organs that do not produce enough photosynthetic products to support their own growth or storage needs

b.) Sources include exporting organs that are capable of producing photosynthate in excess of their own needs

c.) During the growing season of the first year, storage root is a sink, when it accumulates sugars received from the source leaves. During the second growing season the same root becomes a source

d.) Roots, tubers, developing fruits, and immature leaves, which must import carbohydrate for normal development, are all examples of sources tissues

Find the correct combination of the source and sink pattern of translocation.

#Question id: 11807

#Unit 6. System Physiology – Plant

Given below are few statements regarding the cytokinin signalling pathway;

a) Cytokinin inhibits the activation of a Cdc25- like phosphatase, whose role is to remove an inhibitory phosphate group from the Cdc2 kinase

b) Cytokinin oxidase irreversibly inactivates cytokinins, and it could be important in regulating or limiting cytokinin effects

c) Cytokinin signal transduction pathway is completed on multiple places such as ER, Cytosol and Nucleus

d) Elevation of endogenous cytokinin levels in transgenic Arabidopsis results in overexpression of the KNOTTED homeobox transcription factor homologs KNAT1 and STM—genes 

Which of the following combinations  from the given above statements is correct?