To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 10648

#Unit 10. Ecological Principles

Which statements about K are correct?

I) K varies among populations.

II) K varies in space.

III) K varies in time.

IV) K is constant for any given species.

#Question id: 10649

#Unit 10. Ecological Principles

According to the logistic growth equation

A) the number of individuals added per unit time is greatest when N is one fourth of K

B) the per capita growth rate (r) increases as N approaches K

C) population growth is zero when N equals K

D) the population grows exponentially when N approaches K

#Question id: 10650

#Unit 10. Ecological Principles

Following some characteristic feature of K selected species

A. semelparity 

B. Mate choice

C. Density independent mortality

D. large size of offspring

What is the above combination is correct?

#Question id: 10651

#Unit 10. Ecological Principles

Which of the following value of A, B and C in above logistic growth of hypothetical population respectively?

#Question id: 10652

#Unit 10. Ecological Principles

Which factors (limited space or overcrowding; limited food; Predators; parasite and diseased) tend to act in a density-dependent manner; Tony Sinclair (1989) many studies of density dependence in 51 populations of insects, 82 populations of large mammals, and 36 populations of small mammals and birds. Which of the following observation is most likely true?

a) Insects showed a wide variety of causes of density dependence and no one density-dependent factor was of overriding importance

b) The lesser the amount of available food, the greater the mortality of large mammals

c) Space and social interactions were more important for smaller mammals and birds, because they are more territorial

#Question id: 10653

#Unit 10. Ecological Principles

All offspring are produced in a single reproductive event, we term this pattern semelparity. Which of the following is characteristics feature for semelparity individuals?

a) Semelparity is common in insects and other invertebrates

b) Semelparous organisms may live for many years before reproducing

c) semelparity occur only in plant

d) In semelparity, exploitation competition is more common than interference competition