To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 11735

#Unit 6. System Physiology – Plant

In cytokinin biosynthesis is the addition of the isopentenyl side chain from DMAPP to an adenosine moiety. The plant and bacterial IPT enzymes differ substrate used;

a) the bacterial enzyme utilizes AMP

b) the plant enzyme appears to utilize AMP

c) the bacterial enzyme utilizes both ADP and ATP

d) the plant enzyme appears to utilize both ADP and ATP

#Question id: 11736

#Unit 6. System Physiology – Plant

Many plant tissues contain the enzyme cytokinin oxidase, which cleaves the side chain, except

#Question id: 11737

#Unit 6. System Physiology – Plant

Cytokinins have been shown to have effects on many physiological and developmental processes, including____

a) Leaf senescence

b) Apical dominance

c) Gametophytic  development

d) Vascular development

e) Breaking of bud dormancy

f) Causing bud dormancy

#Question id: 11738

#Unit 6. System Physiology – Plant

The ribotide and riboside forms of trans-zeatin can be interconverted, and free trans-zeatin can be formed from the ribotide by the

#Question id: 11739

#Unit 6. System Physiology – Plant

which of the following compound is resist to cleave by cytokinin oxidase and why?

#Question id: 11740

#Unit 6. System Physiology – Plant

Some given characteristics of the two types of sieve elements in seed plants such as Sieve tube elements and Sieve cells. Find out the  true characteristics of sieve tube elements from the given statements;

i) Found in angiosperms

ii) Sieve plate pores are open channels

iii) Albuminous cells sometimes function as companion cells

iv) P-protein is present in all eudicots and many monocots

v) There are no sieve plates; all sieve areas are similar