To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 4807

#Unit 8. Inheritance Biology

Three incompletely link marker gene was found. There is a marker A gene present in middle. Cross between aaBBcc X AAbbCC to produce heterozygous were allowed test cross, which of the following test cross offspring should be least frequent?

#Question id: 4808

#Unit 8. Inheritance Biology

When F1 female Drosophila of the genotype AaBbCc is test crossed, ( Gene A and B located at 10 map unit in transe configuration but gene C are independently assort for both gene. Which of the following test cross progeny that is that is more frequent?

#Question id: 4809

#Unit 8. Inheritance Biology

The upper most following figure above shows the locations of four genes on the genetic map of an organism; the lower figure shows the locations of the same four genes on a physical map derived from the nucleotide sequence of the DNA of that organism. The maps are not identical because

#Question id: 4810

#Unit 8. Inheritance Biology

In a haploid organism, the C and D loci are 8 m.u. apart. From a cross C d X c D, which of the following proportion following progeny classes: (a) C D; (b) c d; (c) C d

#Question id: 4811

#Unit 8. Inheritance Biology

Three incompletely link gene as a B-A-C order. A cross allowed between AAbbcc X aaBBCC produce f1 progeny allowed test cross. Which of the following offspring is a result of double cross over?

#Question id: 4812

#Unit 8. Inheritance Biology

Two character X and Y ,both follow law of dominance due to 3:1 F2 phenotypic ratio monohybrid cross separately If carry out dihybrid selfing cross for heterozygous genotype for both character does not follow law of independence assortment in normal environmental condition due to?