To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

#Question id: 27644

#Unit 1. Molecules and their Interaction Relevant to Biology

#Question id: 27645

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following is correct regarding the β turns

(1) In Type I β turn i+1 lies in RHH region while i+2 lies near BS region 

(2) In Type II β turn i+1 lies in BS while i+2 lies in below LHH

(3) In Type I’ β i+1and i+2 in near LHH which virtually not allowed region for most of amino acid except Gly 

(4) In Type II’ β i+2 lies at 00 psi and i+1 in lower right region both i+2 and   i+1  must be Gly

#Question id: 27664

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following is correctly representing the distance between two successive C α Carbon in a peptide chain

#Question id: 27665

#Unit 1. Molecules and their Interaction Relevant to Biology

There are few statements is given regarding the peptide bond configuration in a chain

(a) The peptide bond is normally (99.6% of the time) in the trans configuration, constraining  to a value of w=+_180

(b) The peptide bond is normally (99.6% of the time) in the Cis configuration, constraining  to a value of w=+_180

(c) In the trans configuration of peptide bond C and C=O lies in the Same plane while in different plane in case of cis configuration 

(d) In the trans configuration of peptide bond C and C=O lies in the different plane while in same plane in case of cis configuration 

Choose which of the following is correct

#Question id: 27666

#Unit 1. Molecules and their Interaction Relevant to Biology

#Question id: 27667

#Unit 1. Molecules and their Interaction Relevant to Biology

There are few statements is given regarding the peptide bond are given

(a) For most peptide bonds the ratio of cis to trans configurations is approximately 1:1000

(b) Peptide bonds formed between Xaa and proline (Xaa is any amino acid) the cis to trans ratio is 1:4

(c) Co and N atoms of the peptide grouping are both in planar sp2 hybridization 

(d) All three kinds of bonds (N-Ca, Ca-Co, and the Co-N peptide bond) are single bonds

(e) The peptide bond is estimated to have 40% double bond character

Choose which of the following is correct