To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 1528

#Unit 4. Cell Communication and Cell Signaling

Mutation of p53 is described as a dominant-negative mutation. Which of the following statement is correct regarding to the mechanism by which this mutation causes the dominant-negative phenotype?

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#Question id: 2069

#Unit 2. Cellular Organization

Which of the following processes includes all of the others?

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#Question id: 23717

#Unit 8. Inheritance Biology

How many phenotypes can be expected from a polygenic inheritance pattern for a character involving eight pairs of polygene?

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#Question id: 27023

#Unit 10. Ecological Principles

when one species uses a second species for housing, is an characteristics of

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#Question id: 24566

#Unit 8. Inheritance Biology

In some sheep, the presence of beard is produced by an autosomal allele that is dominant in males and recessive in females. A beard female is crossed with a homozygous beardless male. One of the resulting F1 females is crossed with a beardless male. What proportion of the male and female progeny from this cross will have beard in F2 generation?