You are practicing designing primers that you can use in PCR reactions. You want your primers to allow you to amplify the sequence found below.

 

 

(a) 5’-ACTTCGATATGTCTAAAATAC-3’ and 5’- CGGTAGCGTCTCTGGTTAGCT -3’

(b) 5’-TGAAGCTATACAGATTTTATG-3’ and 5’-GCCATCGCAGAGACCAATCGA-3’

(c) 5’-GTATTTTAGACATATCGAAGT -3’ and 5’-AGCTAACCAGAGACGCTACCG-3’

You are asked to design a 15-nucleotide-long primer that could potentially hybridize to a portion of a specific mRNA that encodes the protein sequence N-Met-Ala-Tyr-Trp-Pro-C. How many different primers would you have to design in order to ensure that one of them will in fact hybridize along its full length to the mRNA?

TLS Online TPP Program

#Question id: 1693

#Unit 4. Cell Communication and Cell Signaling

The processing of cytosolic protein involves:

TLS Online TPP Program

#Question id: 6572

#General Aptitude

Which term comes next in the sequence : Z, U, Q, ?, L

TLS Online TPP Program

#Question id: 32362

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following reaction is catalyzes by enolase during Glycolysis

TLS Online TPP Program

#Question id: 15904

#Unit 12. Applied Biology

If the cell arc treated with 0.01% solution of FDA, what will be the effect on live cell?

TLS Online TPP Program

#Question id: 7277

#Unit 5. Developmental Biology

According to this model, Which of the following statements are correct?