TLS Online TPP Program

#Question id: 13097


You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
 
If you take any bacterial gene sequence, before you begin doing any sequence analysis on it, there are six potential open reading frames. Why are there six?

#Unit 14. Methods in Biology
  1. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a TGT, a GTT, or a TTC.
  2. There are 6 reading frames on each of two strands because the strand look like the mRNA, then the reading frame could start with a CCC, a CCG, a CGT, a GTA, TAC or a ACG.
  3. There are 3 reading frame only on the top of the strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT.
  4. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a GGG, a GGC, or a GCA.
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TLS Online TPP Program

#Question id: 28691

#Unit 2. Cellular Organization

ER targeting sequence___

TLS Online TPP Program

#Question id: 28692

#Unit 2. Cellular Organization

Which one of the following statements about signal recognition particles (SRPs) is correct?
a) It is a cytosolic ribonucleoprotein
b) It docks with a receptor on the surface of the ER membrane.
c) It is an integral membrane protein
d) It transiently binds to both the ER signal sequence at the C-terminus and the large (60S) ribosomal subunit
e) It acts as a scaffold for the hexamer

TLS Online TPP Program

#Question id: 28693

#Unit 2. Cellular Organization

There are some given statements about co-translational translocation in endoplasmic reticulum

a)SRP and the nascent polypeptide chain–ribosome complex bind to the integral membrane translocon

b)SRP and the nascent polypeptide chain–ribosome complex is strengthened by the binding of GTP to both the SRP and its receptor.

c)ER signal sequence emerges from the ribosome, it is bound by a signal recognition particle (SRP)

d)Hydrolysis of the bound GTP accompanies disassembly of the SRP and SRP receptor

e)Hydrolysis of the bound GTP initiates opening of the translocon, where translocation can take place


Which of the following statement is incorrect?

TLS Online TPP Program

#Question id: 28694

#Unit 2. Cellular Organization

Which one of the following was first identified through mutations in the yeast gene and encoding a protein called Sec61α?

TLS Online TPP Program

#Question id: 28695

#Unit 2. Cellular Organization

O-linked oligosaccharides is representing the attachment of glycoproteins to the hydroxyl (–OH) group in which amino acid residue?

TLS Online TPP Program

#Question id: 28696

#Unit 2. Cellular Organization

N-linked oligosaccharide is attaching carbohydrate chain to the amide nitrogen of which amino acid residue?