You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.

 

You are using shotgun sequencing to determine the DNA sequence of the genome of this new bacterial species. For one strand of a 30-nucleotide long stretch of DNA, you get the following sequences out of your shotgun sequencing reaction. 

 

Choose correct 30-nt-long DNA sequence with the help of above-mentioned fragments

TLS Online TPP Program

#Question id: 23886

#Unit 13. Methods in Biology

In recombinant DNA methods, the term vector can refer to

TLS Online TPP Program

#Question id: 23887

#Unit 13. Methods in Biology

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? 

TLS Online TPP Program

#Question id: 23888

#Unit 13. Methods in Biology

A recessive allele usually results from a mutation that

a. disrupts expression of the gene.

b. alters the structure of the encoded protein leading to gain of function.

c. alters the structure of the encoded protein leading to loss of function.

d. increases the activity of the encoded protein.

TLS Online TPP Program

#Question id: 23889

#Unit 13. Methods in Biology

Temperature-sensitive mutations would be particularly useful for studying which of the following processes?

a. DNA synthesis

b. hormone secretion

c. protein synthesis

d. Mitosis

TLS Online TPP Program

#Question id: 23890

#Unit 13. Methods in Biology

Which of the following is an essential element of plasmids used as cloning vectors?

a. a centromere

b. a telomere

c. an origin of replication

d. a drug resistance gene

TLS Online TPP Program

#Question id: 23891

#Unit 13. Methods in Biology

Which of the following is (are) used both in the polymerase chain reaction (PCR) and in DNA sequencing?

a. single-stranded DNA primers

b. deoxynucleotide triphosphates (dNTPs)

c. dideoxynucleotide triphosphates (ddNTPs)

d. DNA polymerase