With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 Why is it that having more repeats leads to a band that is higher in the gel?

TLS Online TPP Program

#Question id: 34088

#Unit 3. Fundamental Processes

How can the 2D gels identify the DNA intermediates associated with a replication origin? Choose combination of correct statements

A. Y-shaped molecule that has three equal length arms will migrate the most slowly of all such molecules derived from a particular DNA fragment

B. A Y-shaped molecule with two very short replicated arms and a large replicated region will migrate very similarly to the unreplicated version of the same DNA fragment. 

C. Y-shaped molecule that results from the almost completely replicated fragment is similar in shape to a linear molecule two times the size of the unreplicated fragment.

TLS Online TPP Program

#Question id: 34089

#Unit 3. Fundamental Processes

A. Replication Initiation require full methylation of the Dam target sites in the origin

B. Replication Initiation inhibited by hemimethylation of Dam target sites 

C. Replication Initiation inhibited by nonmethylation of Dam target sites 

D. An origin of nonmethylated DNA can function effectively

Choose true and false

TLS Online TPP Program

#Question id: 34090

#Unit 3. Fundamental Processes

A. DnaA is in the active form only when bound to ATP.

B. All three13-bp repeats of origin must be opened for the initiation

C. 9-bp repeats are found outside of 245 bp origin 

D. DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Had

Choose true and false

TLS Online TPP Program

#Question id: 34091

#Unit 3. Fundamental Processes

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends. This ATPase activity is stimulated by 

A. Membrane phospholipids which helps in ATP hydrolysis

B. dsDNA which is substrate for DnaA

C. Membrane phospholipids which provide ATP in exchange of ADP

D. ssDNA which is created in first step

Which of the following is correct combination

TLS Online TPP Program

#Question id: 34092

#Unit 3. Fundamental Processes

A. SeqA binds to hemimethylated DNA and is required for delaying rereplication.

B. As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases.

C. The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

D. Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

Choose true and false

TLS Online TPP Program

#Question id: 34093

#Unit 3. Fundamental Processes

Quite large regions of the eukaryotic chromosome can be characterized as “early replicating” or “late replicating,”. These site can be recognised by