TLS Online TPP Program
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TLS Online TPP Program
#Question id: 39278
#Plant Biotechnology
Q. Consider the following statements regarding cytoplasm transfer via somatic hybridization:
Statement I: Cytoplasm transfer that normally takes 6–7 years via backcrossing can be achieved in just one year using protoplast fusion.
Statement II: Iodoacetate is used to inactivate the nucleus of the recipient parent protoplasts.
Statement III: Irradiation of donor protoplasts is used to inactivate their nuclei.
Statement IV: The resulting cybrids contain the nucleus of parent A and mitochondria of parent B.
Which of the statements are correct?
TLS Online TPP Program
#Question id: 39279
#Plant Biotechnology
Q. Regarding the tomato × Solanum acaule cybrids:
Statement I: Regenerants were morphologically and cytologically similar to tomato.
Statement II: They exhibited cytoplasmic male sterility (CMS) inherited maternally.
Statement III: CMS expression disappeared after one generation.
Statement IV: Some regenerants had malformed or nonfunctional pollen.
Which statements are correct?
TLS Online TPP Program
#Question id: 39280
#Plant Biotechnology
Q. Regarding cybridization for male sterile line development:
Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.
Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.
Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.
Which statements are correct?
TLS Online TPP Program
#Question id: 39281
#Plant Biotechnology
Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization.
Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.
TLS Online TPP Program
#Question id: 39282
#Plant Biotechnology
Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A).
Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.
TLS Online TPP Program
#Question id: 39283
#Plant Biotechnology
Q. Statement I: In the fusion product described, the mitochondria are always derived from the donor strain (Parent B).
Statement II: The chloroplasts in the resulting regenerants must always come from the recipient strain (Parent A)