In plant tissue culture:

1. High cytokinin: low auxin →

2. High auxin: low cytokinin

TLS Online TPP Program

#Question id: 37924

#Plant Biotechnology

Which of the following statements about the mechanical method of protoplast isolation is INCORRECT?

TLS Online TPP Program

#Question id: 37925

#Plant Biotechnology

Which type of cells is NOT suitable for protoplast isolation by the mechanical method?

TLS Online TPP Program

#Question id: 37926

#Plant Biotechnology

Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

 B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

 C. Surface sterilization and epidermis removal from leaf explant

 D. Density gradient centrifugation to separate viable protoplasts

 E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

TLS Online TPP Program

#Question id: 37927

#Plant Biotechnology

Protoplasts require osmotic stabilizers in culture media because they:

TLS Online TPP Program

#Question id: 37928

#Plant Biotechnology

Which of the following is a non-ionic osmotic stabilizer commonly used in protoplast culture?

TLS Online TPP Program

#Question id: 37929

#Plant Biotechnology

Mannitol is preferred for leaf mesophyll protoplasts because it is: