Consider the following statements about plant tissue culture techniques and their applications:

(A) Meristem culture is used for virus-free plant production.

(B) Seed culture increases efficiency of seed germination.

(C) Callus culture is used to produce somatic embryos.

(D) Anther culture and ovary culture both produce haploid plants.

(E) Suspension culture is mainly used for producing multicellular tissue masses.

(F) Protoplast culture is applied for somatic hybrid production.

Which of the following combinations of statements is correct?

TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.

Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.

TLS Online TPP Program

#Question id: 38776

#Plant Biotechnology

Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.

Statement II: Calcium ions improve plasma membrane stability of protoplasts.

TLS Online TPP Program

#Question id: 38777

#Plant Biotechnology

Q. Statement I: B5 and MS media are commonly used for culturing isolated protoplasts.

Statement II: These media provide essential macro- and micronutrients required for protoplast survival and division.

TLS Online TPP Program

#Question id: 38778

#Plant Biotechnology

Q. Statement I: Mannitol and sorbitol are preferred osmotic stabilizers during protoplast isolation.

Statement II: These compounds are metabolized rapidly by protoplasts to generate energy.

TLS Online TPP Program

#Question id: 38779

#Plant Biotechnology

Q. Assertion (A): Vegetatively propagated crops are more prone to rapid spread of viral diseases.

Reason (R): Vegetative propagules directly transmit virus particles from the infected parent plant.

Choose the correct option:

TLS Online TPP Program

#Question id: 38780

#Plant Biotechnology

Q. Statement I: Sexual reproduction generally limits virus transmission in plants.

Statement II: Many plant viruses are now known to be seed-transmissible.

Choose the correct option