Which microscopy can be used for viewing live cells and unstained tissues?

1. Fluorescence microscopy

2. Phase contrast microscopy

3. Dark field microscopy

4. Differential interference microscopy

5. Confocal microscopy

Which of the above combinations of microscopy is correct?

TLS Online TPP Program

#Question id: 38769

#Plant Biotechnology

Q. Statement I: During the washing step, PEG may remove plasma membrane components bound to it.

Statement II: Removal of these components can destabilize membrane integrity and promote fusion of adjacent protoplasts.

TLS Online TPP Program

#Question id: 38770

#Plant Biotechnology

Q. Statement I: PEG-induced fusion is a consequence of controlled membrane damage and reorganization.

Statement II: Plasma membrane destabilization increases the likelihood of fusion between closely placed protoplasts.

TLS Online TPP Program

#Question id: 38771

#Plant Biotechnology

Q. Statement I: PEG can interact with both negatively and positively charged components of the plasma membrane.

Statement II: Such interactions reduce electrostatic repulsion between protoplasts.

TLS Online TPP Program

#Question id: 38772

#Plant Biotechnology

Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.

Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.

TLS Online TPP Program

#Question id: 38773

#Plant Biotechnology

Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.

Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.