Why is ploidy analysis considered an essential step after anther culture?

TLS Online TPP Program

#Question id: 39289

#Plant Biotechnology

Q. Assertion:Brassica napus lines carrying Ogura (radish) cytoplasm are highly efficient for commercial growth in cold climates without any modifications. 

Reason: The presence of radish chloroplasts in these lines causes chlorophyll deficiency under low temperatures.

TLS Online TPP Program

#Question id: 39290

#Plant Biotechnology

Q. Assertion: Protoplast fusion between CMS B. napus (with radish cytoplasm) and normal B. napus can produce healthy male sterile plants. 

Reason: The resulting cybrids can retain the radish mitochondria (for CMS) while replacing the radish chloroplasts with B. napus chloroplasts.

TLS Online TPP Program

#Question id: 39291

#Plant Biotechnology

Q. Assertion: In plant breeding, cytoplasm transfer via somatic hybridization is significantly faster than conventional backcrossing. 

Reason: Protoplast fusion can achieve in one year what typically takes 5–6 years through the backcrossing method.

TLS Online TPP Program

#Question id: 39292

#Plant Biotechnology

Q. Assertion: Symmetric hybrids allow for the transfer of genes between species that are sexually incompatible. 

Reason: Somatic hybridization bypasses the requirements of pollination and fertilization, enabling fusion between even completely sterile lines like monoploids.

TLS Online TPP Program

#Question id: 39293

#Plant Biotechnology

Q. Assertion: Cybridization enables the creation of unique organelle combinations that cannot be produced through sexual reproduction. 

Reason: Somatic fusion allows the mitochondria of one species to be combined with the chloroplasts of another, often resulting in recombinant organellar genomes. 

TLS Online TPP Program

#Question id: 39294

#Plant Biotechnology

Q. Assertion: Somatic hybridization is currently the primary method used for the commercial improvement of all major cereal and pulse crops. 

Reason: Techniques for protoplast isolation, culture, and fusion are not yet readily available for many important cereals and pulses.