Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 38068

#Plant Biotechnology

Which of the following explants is Classically associated with Indirect Somatic Embryogenesis in Daucus carota (Carrot)? 

TLS Online TPP Program

#Question id: 38069

#Plant Biotechnology

Match the Terminology (List I) with its Description/Example (List II):

List I (Terminology)	List II (Description/Example)
A. PEDC	I. Cells requiring induction to re-enter mitotic cycle & form callus
B. IEDC	II. Leaf tissue of Coffee
C. Direct Embryogenesis	III. Cells already committed to embryonic development
D. Indirect Embryogenesis	IV. Scutellum of Barley/Rice

Choose the correct option: 

TLS Online TPP Program

#Question id: 38070

#Plant Biotechnology

Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A): Direct somatic embryogenesis is generally rarer than indirect somatic embryogenesis.

Reason (R): Direct embryogenesis occurs from "Pre-Embryonic Determined Cells" (PEDCs) which are found only in specific embryonic tissues like the scutellum or nucellus.

In light of the above statements, choose the correct answer: 

TLS Online TPP Program

#Question id: 38071

#Plant Biotechnology

Given below are two statements:

Statement I: In Indirect Somatic Embryogenesis, the explant first proliferates into unorganized callus tissue before differentiating into embryos.

Statement II: IEDCs (Induced Embryogenic Determined Cells) need only a "permissive" signal to start embryogenesis and do not require dedifferentiation.

Choose the most appropriate answer: 

TLS Online TPP Program

#Question id: 38072

#Plant Biotechnology

Based on the "Origin" characteristic, which of the following correctly distinguishes a somatic embryo from a shoot bud? 

TLS Online TPP Program

#Question id: 38073

#Plant Biotechnology

Which structure is described as "Bipolar," possessing both shoot and root poles?