TLS Online TPP Program

#Question id: 13052


What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

#Biochemistry
  1. PCR would proceed normally
  2. Non-specific PCR of random templates will occur
  3. Non-specific PCR of random templates will occur
  4. The PCR reaction will not commence
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TLS Online TPP Program

#Question id: 39259

#Plant Biotechnology

Q. Assertion (A): After repeated backcrossing of asymmetric hybrids with N. plumbaginifolia, plants with a diploid N. plumbaginifolia genome and a few A. belladonna chromosomes were recovered.
Reason (R): Backcrossing helps eliminate unwanted alien chromosomes while retaining selected genes.

TLS Online TPP Program

#Question id: 39260

#Plant Biotechnology

Q. Assertion (A): All asymmetric hybrids contained nitrate reductase gene and rDNA repeats of Atropa belladonna.
Reason (R): rDNA repeats are often preferentially retained during asymmetric hybridization.

TLS Online TPP Program

#Question id: 39261

#Plant Biotechnology

Q.Assertion (A): Hybrids obtained from irradiated carrot and non-irradiated rice protoplast fusion resembled rice plants.
Reason (R): The non-irradiated parent usually contributes more intact genomic material.

TLS Online TPP Program

#Question id: 39262

#Plant Biotechnology

Q. Assertion: The fusion of B. napus (AACC) and B. nigra (BB) results in a hybrid with the genomic constitution AABBCC. 
Reason: This specific interspecific fusion was primarily conducted to transfer resistance against Phoma lingam (black-leg disease) to rapeseed.
 

TLS Online TPP Program

#Question id: 39263

#Plant Biotechnology

Q. Assertion: Somatic hybridization between non-flowering and male sterile dihaploid clones of potato can result in male fertile hybrids. 
Reason: Symmetric hybridization allows for the creation of superior hybrids and the generation of novel genetic material by bypassing traditional sexual reproduction barriers.

TLS Online TPP Program

#Question id: 39264

#Plant Biotechnology

Q. Assertion: Tobacco hybrids produced from N. rustica and N. tabacum may exhibit resistance to blue mould. 
Reason: Blue mould resistance was a primary trait present in both parent lines and was intentionally selected for during the initial fusion process.