TLS Online TPP Program

#Question id: 38096


Selection of resistant cell lines using somaclonal variation involves:

#Plant Biotechnology
  1. Regeneration before inhibitor exposure
  2. Exposure of callus/cells to an inhibitor followed by regeneration
  3. Screening before plant regeneration
  4. Direct seed treatment with inhibitors
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TLS Online TPP Program

#Question id: 37835

#Plant Biotechnology

Which statement is TRUE about plant cellular totipotency?

TLS Online TPP Program

#Question id: 37836

#Plant Biotechnology

Column AColumn B
1. Dry HeatA. 121 °C at 15 PSI for 15–20 min
2. AutoclavingB. 0.45 μm pore size membrane
3. Filter SterilizationC. 160–180°C for 3 hours
4. Laminar Air FlowD. HEPA filter (removes particles > 0.3 μm)

TLS Online TPP Program

#Question id: 37837

#Plant Biotechnology

Column A (Method)Column B (Material to Sterilize)
1. Surface SterilizationA. Heat labile growth regulators (GA, Zeatin)
2. Filter SterilizationB. Glassware and Teflon plastic ware
3. Dry HeatC. Plant materials (explants, ovules)
4. Wiping with 70% EthanolD. Hands of operator and cabinet platform

TLS Online TPP Program

#Question id: 37838

#Plant Biotechnology

Column A (Process)Column B (Chemical/Agent Used)
1. Surface SterilizationA. 95% Alcohol (followed by flaming)
2. Flame SterilizationB. Sodium Hypochlorite / Mercuric Chloride
3. Cleaning Laminar PlatformC. 70% Ethyl Alcohol
4. Air FiltrationD. HEPA (High Efficiency Particulate Air)

TLS Online TPP Program

#Question id: 37839

#Plant Biotechnology

Column AColumn B
1. HEPA FilterA. Used for heat-labile vitamins & enzymes
2. AutoclaveB. Filters out particles > 0.3 µm
3. Membrane Filter (0.45 μm)C. Sterilizes culture vessels using steam pressure
4. HgCl₂ (Mercuric Chloride)D. Agent used for surface sterilization of explants

TLS Online TPP Program

#Question id: 37840

#Plant Biotechnology

In plant tissue culture:
1. High cytokinin: low auxin →
2. High auxin: low cytokinin