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TLS Online TPP Program

#Question id: 10363

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?

#Applied Microbiology
  1. A-ii, B-v, C-iv, D-iii, E-i  
  2. A-iii, B-v, C-i, D-ii, E-iv
  3. A-iv, B-iii, C-i, D-ii, E-v      
  4. A-i, B-ii, C-iii, D-v, E-iv
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TLS Online TPP Program

#Question id: 40198

#Applied Microbiology

Read the following statements regarding Fed-Batch culture and choose the correct option.

Statement I: Fed-batch culture is ideal for producing secondary metabolites (like antibiotics) because it allows the culture to be maintained in the stationary phase for a prolonged period.

Statement II: In fed-batch culture, the volume of the culture broth remains constant throughout the fermentation process. 
TLS Online TPP Program

#Question id: 40199

#Applied Microbiology

Match the parameter in Column A with its formula or definition in Column B

Column A (Parameter)Column B (Definition/Formula)
1. Dilution Rate (D)A. ln (2) / mu
2. Doubling Time (td)B. Flow rate (F) / Volume (V)
3. Yield Coefficient (Y)C. Change in cell mass / Change in substrate consumed
4. Specific Growth Rate (mu)D. 1/x . dx/dt
TLS Online TPP Program

#Question id: 40200

#Applied Microbiology

Calculate the melting temperature (Tm) for the following primer sequence using the Wallace Rule

Sequence: 5'-GGCCAAAATTTTGGCC-3'
TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation
TLS Online TPP Program

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine
TLS Online TPP Program

#Question id: 40203

#Applied Microbiology

Which cofactor is essential for the activity of Taq DNA polymerase during the PCR extension step?